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Rabbit anti-FUS Recombinant Monoclonal Antibody [BLR023E]

Catalogue number:
A700-023-T
Size:
20 µl (2 blots)
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£137.00 Estimated delivery Wed 15 Oct
Shipping is calculated in checkout
Applications:

WB, IP, IHC, IHC-IF, ICC-IF

Antibody Host:

Rabbit

Target:

FUS

Species Reactivity:

Human; Mouse

Antibody Type:

Recombinant Monoclonal

Immunogen:

Between 1 and 50

Alternative Names:

TLS; HNRNPP2; oncogene FUS; translocated in liposarcoma protein; oncogene TLS; FUS/ERG chimeric protein; RNA-binding protein FUS; heterogeneous nuclear ribonucleoprotein P2; POMP75; fusion gene in myxoid liposarcoma; fused in sarcoma; FUS/ERG fusion prote

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Product Description:

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide

Detection of human and mouse FUS by west
Detection of human and mouse FUS by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FUS recombinant monoclonal antibody [BLR023E] (A700-023-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds.
Detection of human FUS by western blot o
Detection of human FUS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-FUS recombinant monoclonal antibody [BLR023E] (A700-023-T lot 1) used for IP at 20 µl per reaction. FUS was also immunoprecipitated by rabbit anti-FUS antibodies (A300-302A, A300-293A). For blotting immunoprecipitated FUS, A700-023-T was used at 1:1000. Chemiluminescence with an exposure time of 3 minutes.