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Rabbit anti-Granzyme B Recombinant Monoclonal Antibody [BLR022E]

Catalogue number:
A700-022-T
Size:
10 µl (5+ tests) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£128.00 Estimated delivery Wed 24 Apr
Shipping is calculated in checkout
Applications:

WB, IHC, ICC, IHC-IF, Flow Cyt, mIF

Antibody Host:

Rabbit

Target:

Granzyme B

Species Reactivity:

Human

Antibody Type:

Recombinant Monoclonal

Immunogen:

residues 19-247 (mature protein)

Alternative Names:

SECT; fragmentin 2; T-cell serine protease 1-3E; granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); Granzyme-2; HLP; Lymphocyte protease; cytotoxic T-lymphocyte proteinase 2; human lymphocyte protein; CGL1; cytotoxic

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Product Description:

Rabbit anti-Granzyme B Recombinant Monoclonal Antibody [BLR022E] - 10 µl (5+ tests)

Storage Temperature:

2 - 8°C

Detection of human Granzyme B by immunoh
Detection of human Granzyme B by immunohistochemistry. Sample: FFPE section of human metastatic lymph node. Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody [BLR022E] (A700-022-T lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
Detection of human Granzyme B by immunoc
Detection of human Granzyme B by immunocytochemistry. Sample: FFPE sections of Granzyme B over-expressing cells (left) and Granzyme A over-expressing cells (right). Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody [BLR022E] (A700-022-T lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
Detection of human Granzyme B by western
Detection of human Granzyme B by western blot. Samples: myc-tagged Granzyme A, H, and B overexpressing cell lysate (15 µg) prepared using NETN lysis buffer. Antibodies: Rabbit anti-Granzyme B recombinant monoclonal antibody [BLR022E] (A700-022-T lot 1) used at 1:1000. Lower Panel: Anti-myc antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.
Detection of human Granzyme B by western
Detection of human Granzyme B by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from SR cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Granzyme B recombinant monoclonal antibody [BLR022E] (A700-022 lot 1) used for WB at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.
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