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  2. Bethyl Laboratories, Inc.
  3. A700-019-T
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Rabbit anti-PBRM1 Recombinant Monoclonal Antibody [BL-39-2C3]

Catalogue number:
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A700-019-T
Supplier:
Bethyl Laboratories, Inc.
Size:
20 µl (2 blots) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£128.00
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Estimated delivery Fri 10 May
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Applications:

WB, IP, IHC, ICC, IHC-IF, Flow Cyt, SW-Size

Antibody Host:

Rabbit

Target:

PBRM1

Species Reactivity:

Human; Mouse

Antibody Type:

Recombinant Monoclonal

Immunogen:

residues 1-293

Alternative Names:

BAF180; protein polybromo-1; polybromo-1D; PB1; BRG1-associated factor 180; hPB1

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Product Description:

Rabbit anti-PBRM1 Recombinant Monoclonal Antibody [BL-39-2C3] - 20 µl (2 blots)

Storage Temperature:

2 - 8°C

Detection of human PBRM1 by western blot
Detection of human PBRM1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (A700-019-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds.
Detection of human PBRM1 by western blot
Detection of human PBRM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (A700-019-T lot 1) used for IP at 20 µl per reaction. For blotting immunoprecipitated PBRM1, A700-019 was used at 1:1000. Chemiluminescence with an exposure time of 3 minutes.
Detection of human PBRM1 by immunohistoc
Detection of human PBRM1 by immunohistochemistry. Sample: FFPE section of human breast. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (A700-019-T lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
Detection of human PBRM1 by immunocytoch
Detection of human PBRM1 by immunocytochemistry. Sample: FFPE section of Jurkat cells. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (A700-019-T lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
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