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Rabbit anti-SMC1 Recombinant Monoclonal Antibody [BL-205-2G8]

Catalogue number:
A700-018-T
Size:
20 µl (2 blots) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£128.00 Estimated delivery Wed 3 Apr
Shipping is calculated in checkout
Applications:

WB, IP, IHC, ICC, IHC-IF, Flow Cyt

Antibody Host:

Rabbit

Target:

SMC1

Species Reactivity:

Human; Mouse

Antibody Type:

Recombinant Monoclonal

Immunogen:

between 1200 to 1233 (C-term)

Alternative Names:

SMC protein 1A; SMC-1A; structural maintenance of chromosomes protein 1A; SMCB; SMC1L1; SMC-1-alpha; SMC1alpha; SMC1; segregation of mitotic chromosomes 1; SB1.8; epididymis secretory sperm binding protein; DXS423E; CDLS2; SMC1 (structural maintenance of

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Product Description:

Rabbit anti-SMC1 Recombinant Monoclonal Antibody [BL-205-2G8] - 20 µl (2 blots)

Storage Temperature:

2 - 8°C

Detection of human and mouse SMC1 by wes
Detection of human and mouse SMC1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, NIH 3T3, and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (A700-018-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.
Detection of human SMC1 by western blot
Detection of human SMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-155-1C11] (A700-018-T lot 1) used for IP at 20 µl per reaction. SMC1 was also immunoprecipitated by rabbit anti-SMC1 antibody A300-055A. For blotting immunoprecipitated SMC1, A700-018 was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.
Detection of human SMC1 by immunohistoch
Detection of human SMC1 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (A700-018-T lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
Detection of human SMC1 by immunocytoche
Detection of human SMC1 by immunocytochemistry. Sample: FFPE section of HCT 116 cells. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (A700-018-T lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
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