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Rabbit anti-CD247/CD3Z Recombinant Monoclonal Antibody [BL-336-1B2]

Catalogue number:
A700-017-T
Size:
10 µl (5+ tests) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£128.00 Estimated delivery Fri 5 Apr
Shipping is calculated in checkout
Applications:

WB, IP, IHC, IHC-IF, Flow Cyt, SW-Size

Antibody Host:

Rabbit

Species Reactivity:

Human

Antibody Type:

Recombinant Monoclonal

Immunogen:

between 150 to 164 (C-term)

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Product Description:

Rabbit anti-CD247/CD3Z Recombinant Monoclonal Antibody [BL-336-1B2] - 10 µl (5+ tests)

Storage Temperature:

2 - 8°C

Detection of CD247/CD3Z in FFPE metastat
Detection of CD247/CD3Z in FFPE metastatic lymph node from lung cancer origin by immunohistochemistry. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (A700-017-T lot 1) used at 1:500. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Detection of human CD247/CD3Z by immunoh
Detection of human CD247/CD3Z by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (Cat. No. A700-017-T Lot1) used 1:500. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Detection of human CD247/CD3Z by western
Detection of human CD247/CD3Z by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, MOLT-4, K-562, SR, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (A700-017-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.
Detection of human CD247/CD3Z by western
Detection of human CD247/CD3Z by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (A700-017-T lot 1) used for IP at 20 µl per reaction. CD247/CD3Z was also immunoprecipitated by rabbit anti-CD247/CD3Z antibodies (A305-170A, A305-212A). For blotting immunoprecipitated CD247/CD3Z, A700-017-T was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.
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