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  3. A700-014-T
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Rabbit anti-KAP-1 Recombinant Monoclonal Antibody [BL-248-2G6]

Catalogue number:
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A700-014-T
Supplier:
Bethyl Laboratories, Inc.
Size:
20 µl (2 blots) _$$_
Product is available in:
  • UK
  • Ireland
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£128.00
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Estimated delivery Fri 26 Apr
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Applications:

WB, IP, IHC, ICC, Flow Cyt

Antibody Host:

Rabbit

Target:

KAP-1

Species Reactivity:

Human; Mouse

Antibody Type:

Recombinant Monoclonal

Immunogen:

surrounding serine 824

Alternative Names:

KRAB [Kruppel-associated box domain]-associated protein 1; RING finger protein 96; Tripartite motif-containing protein 28; transcriptional intermediary factor 1-beta; transcription intermediary factor 1-beta; TIF1-beta; TIF1B; TF1B; RNF96; RING-type E3 ub

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Product Description:

Rabbit anti-KAP-1 Recombinant Monoclonal Antibody [BL-248-2G6] - 20 µl (2 blots)

Storage Temperature:

2 - 8°C

Detection of human and mouse KAP-1 by we
Detection of human and mouse KAP-1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, NIH 3T3, and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (A700-014-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.
Detection of human KAP-1 by western blot
Detection of human KAP-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells that were treated with 100 µM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (A700-014-T lot 1) used for IP at 20 µl per reaction. KAP-1 was also immunoprecipitated by rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (A700-013). For blotting immunoprecipitated KAP-1, A700-014-T was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds.
Detection of human KAP-1 by immunohistoc
Detection of human KAP-1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (A700-014-T lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
Detection of human KAP-1 by immunocytoch
Detection of human KAP-1 by immunocytochemistry. Sample: FFPE section of HeLa cells. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (A700-014-T lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
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