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  3. A700-014-T
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Rabbit anti-KAP-1 Recombinant Monoclonal Antibody [BL-248-2G6]

Catalogue number:
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A700-014-T
Supplier:
Bethyl Laboratories, Inc.
Size:
20 µl (2 blots)
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£137.00
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Estimated delivery Fri 28 Nov
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Applications:

Flow Cyt,ICC,IHC,IP,WB

Antibody Host:

Rabbit

Target:

KAP-1

Species Reactivity:

Human;Mouse

Immunogen:

Surrounding serine 824

Alternative Names:

E3 SUMO-protein ligase TRIM28; KAP1; KAP-1; KRAB Kruppel-associated box domain-associated protein 1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP-1; nuclear corepressor KAP-1; PPP1R157; protein phosphatase 1, regulatory subunit 157; RING fi

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Product Description:

Rabbit anti-KAP-1 Recombinant Monoclonal Antibody [BL-248-2G6] - 20 µl (2 blots)

Detection of human and mouse KAP-1 by we
Detection of human and mouse KAP-1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, NIH 3T3, and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (A700-014-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.
Detection of human KAP-1 by western blot
Detection of human KAP-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells that were treated with 100 µM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (A700-014-T lot 1) used for IP at 20 µl per reaction. KAP-1 was also immunoprecipitated by rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (A700-013). For blotting immunoprecipitated KAP-1, A700-014-T was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds.