www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

loading...
loading...
  1. home
  2. Bethyl Laboratories, Inc.
  3. A700-013-T
*

Rabbit anti-Phospho KAP-1 (S824) Recombinant Monoclonal Antibody [BL-246-7B5]

Catalogue number:
Add to favourites
A700-013-T
Supplier:
Bethyl Laboratories, Inc.
Size:
20 µl (2 blots)
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£137.00
add to basket
Estimated delivery Fri 28 Nov
Shipping is calculated in checkout
Applications:

Flow Cyt,ICC-IF,IHC,IP,WB

Antibody Host:

Rabbit

Target:

KAP-1 Phospho (S824)

Species Reactivity:

Human;Mouse

Immunogen:

Surrounding serine 824

Alternative Names:

E3 SUMO-protein ligase TRIM28; KAP1; KAP-1; KRAB Kruppel-associated box domain-associated protein 1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP-1; nuclear corepressor KAP-1; PPP1R157; protein phosphatase 1, regulatory subunit 157; RING fi

image 0
image 1
Product Description:

Rabbit anti-Phospho KAP-1 (S824) Recombinant Monoclonal Antibody [BL-246-7B5] - 20 µl (2 blots)

Detection of human Phospho KAP-1 (S824)
Detection of human Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (15 µg) from HEK293T cells treated with 100 µM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (A700-013-T lot 1) used at 1:1000. Lower panel shows western blot for total KAP-1 using rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (A700-014) at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds.
Detection of human Phopsho KAP-1 (S824)
Detection of human Phopsho KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells that were treated with 100 µM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (A700-013-T lot 1) used for IP at 20 µl per reaction. KAP-1 was also immunoprecipitated by rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (A700-014). For blotting immunoprecipitated KAP-1, A700-013-T was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.