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  3. A700-013-T
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Rabbit anti-Phospho KAP-1 (S824) Recombinant Monoclonal Antibody [BL-246-7B5]

Catalogue number:
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A700-013-T
Supplier:
Bethyl Laboratories, Inc.
Size:
20 µl (2 blots) _$$_
Product is available in:
  • UK
  • Ireland
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£128.00
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Estimated delivery Fri 3 May
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Applications:

WB, IP, IHC, ICC-IF, Flow Cyt, SW-Size

Antibody Host:

Rabbit

Target:

KAP-1, Phospho (S824)

Species Reactivity:

Human

Antibody Type:

Recombinant Monoclonal

Immunogen:

surrounding serine 824

Alternative Names:

KRAB [Kruppel-associated box domain]-associated protein 1; RING finger protein 96; Tripartite motif-containing protein 28; transcriptional intermediary factor 1-beta; transcription intermediary factor 1-beta; TIF1-beta; TIF1B; TF1B; RNF96; RING-type E3 ub

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Product Description:

Rabbit anti-Phospho KAP-1 (S824) Recombinant Monoclonal Antibody [BL-246-7B5] - 20 µl (2 blots)

Storage Temperature:

2 - 8°C

Detection of human Phospho KAP-1 (S824)
Detection of human Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (15 µg) from HEK293T cells treated with 100 µM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (A700-013-T lot 1) used at 1:1000. Lower panel shows western blot for total KAP-1 using rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (A700-014) at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds.
Detection of human Phopsho KAP-1 (S824)
Detection of human Phopsho KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells that were treated with 100 µM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (A700-013-T lot 1) used for IP at 20 µl per reaction. KAP-1 was also immunoprecipitated by rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (A700-014). For blotting immunoprecipitated KAP-1, A700-013-T was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.
Detection of human Phospho KAP-1(S824) b
Detection of human Phospho KAP-1(S824) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (A700-013-T lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
Detection of human Phospho KAP-1 (S824)
Detection of human Phospho KAP-1 (S824) by immunocytochemistry. Samples: Formaldehyde-fixed asynchronous HeLa cells grown in chambered microscope slides and treated with etoposide (right) or untreated (left). Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (A700-013-T lot 1) used at of 1:100. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG (A120-201D4). Counterstain: Phalloidin conjugated Alexa Fluor® 488 (green).
Detection of human Phospho Kap1 (S824) b
Detection of human Phospho Kap1 (S824) by immunohistochemistry and immunocytochemistry. Samples: FFPE serial sections of breast carcinoma and FFPE serial sections of etoposide treated HeLa cells. Mock phosphatase treated sections (left) and calf intestinal phosphatase-treated sections (right). Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (A700-013-T lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
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