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  2. Bethyl Laboratories, Inc.
  3. A700-012-T
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Rabbit anti-CD45 Recombinant Monoclonal Antibody [BL-178-12C7]

Catalogue number:
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A700-012-T
Supplier:
Bethyl Laboratories, Inc.
Size:
10 µl (5+ tests) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£128.00
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Estimated delivery Fri 26 Apr
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Applications:

WB, IP, IHC, ICC, IHC-IF, Flow Cyt

Antibody Host:

Rabbit

Target:

CD45

Species Reactivity:

Human

Antibody Type:

Recombinant Monoclonal

Immunogen:

between 1250 to 1306 (C-term)

Alternative Names:

L-CA; protein tyrosine phosphatase, receptor type, c polypeptide; T200 leukocyte common antigen; T200 glycoprotein; receptor-type tyrosine-protein phosphatase C; LY5; Leukocyte common antigen; GP180; CD45R; B220; CD45 antigen; LCA; CD45; CD antigen CD45;

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Product Description:

Rabbit anti-CD45 Recombinant Monoclonal Antibody [BL-178-12C7] - 10 µl (5+ tests)

Storage Temperature:

2 - 8°C

Detection of human CD45 by immunohistoch
Detection of human CD45 by immunohistochemistry. Sample: FFPE section of human appendix. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (A700-012-T lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
Detection of human CD45 by immunocytoche
Detection of human CD45 by immunocytochemistry. Sample: FFPE section of Jurkat cells. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (A700-012-T Lot 1) used at a dilution of 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
Detection of human CD45 by immunohistoch
Detection of human CD45 by immunohistochemistry. Sample: FFPE section of human small intestine. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (A700-012-T lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
Detection of human CD45 by immunhistoche
Detection of human CD45 by immunhistochemistry. Sample: FFPE section of appendix. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (A700-012-T lot 1) used at 1:250. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG (A120-101D4).
Detection of human CD45 by western blot.
Detection of human CD45 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, MOLT-4, K-562, SR, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (A700-012-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 seconds.
Detection of human CD45 by western blot
Detection of human CD45 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (A700-012-T lot 1) used for IP at 20 µl per reaction. CD45 was also immunoprecipitated by rabbit anti-CD45 antibody (A304-376A). For blotting immunoprecipitated CD45, A700-012-T was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds.
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