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  3. A700-011-T
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Rabbit anti-53BP1 Recombinant Monoclonal Antibody [BL-250-1H11]

Catalogue number:
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A700-011-T
Supplier:
Bethyl Laboratories, Inc.
Size:
20 µl
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£113.60
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Estimated delivery Fri 11 Sep
Special price until 01 Dec 2026
Normal price £142.00
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Applications:

FC, IHC, IP, WB

Antibody Host:

Rabbit

Target:

53BP1

Species Reactivity:

Human;Mouse

Antibody Isotype:

IgG

Antibody Type:

Primary Antibody

Immunogen:

Between 350 and 400

Alternative Names:

53BP1;p202;p53-binding protein 1;p53BP1;TDRD30;TP53-binding protein 1;tumor protein 53-binding protein, 1;tumor suppressor p53-binding protein 1

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Product Description:

Rabbit anti-53BP1 Recombinant Monoclonal Antibody [BL-250-1H11] - 20 µl

Detection of human 53BP1 by western blot
Detection of human 53BP1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (A700-011-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 second.
Detection of human 53BP1 by western blot
Detection of human 53BP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (A700-011-T lot 1) used for IP at 20 µl per reaction. 53BP1 was also immunoprecipitated by rabbit anti-53BP1 antibody (A300-272A). For blotting immunoprecipitated 53BP1, A700-011-T was used at 1:1000. Substrate: Chemiluminescence with an exposure time of 10 second.