www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

loading...
loading...
  1. home
  2. Bethyl Laboratories, Inc.
  3. A700-011-T
*

Rabbit anti-53BP1 Recombinant Monoclonal Antibody [BL-250-1H11]

Catalogue number:
Add to favourites
A700-011-T
Supplier:
Bethyl Laboratories, Inc.
Size:
20 µl (2 blots)
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£137.00
add to basket
Estimated delivery Wed 15 Oct
Shipping is calculated in checkout
Applications:

WB, IP, IHC, Flow Cyt

Antibody Host:

Rabbit

Target:

53BP1

Species Reactivity:

Human; Mouse

Antibody Type:

Recombinant Monoclonal

Immunogen:

Between 350 and 400

Alternative Names:

p53-binding protein 1; tumor suppressor p53-binding protein 1; tumor protein 53-binding protein, 1; TP53-binding protein 1; p53BP1; p202; 53BP1; TDRD30

image 0
image 1
Product Description:

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide

Detection of human 53BP1 by western blot
Detection of human 53BP1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (A700-011-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 second.
Detection of human 53BP1 by western blot
Detection of human 53BP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (A700-011-T lot 1) used for IP at 20 µl per reaction. 53BP1 was also immunoprecipitated by rabbit anti-53BP1 antibody (A300-272A). For blotting immunoprecipitated 53BP1, A700-011-T was used at 1:1000. Substrate: Chemiluminescence with an exposure time of 10 second.