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  3. A700-009-T
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Rabbit anti-Phospho RPA32 (S4/S8) Recombinant Monoclonal Antibody [BL-165-5F1]

Catalogue number:
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A700-009-T
Supplier:
Bethyl Laboratories, Inc.
Size:
20 µl (2 blots) _$$_
Product is available in:
  • UK
  • Ireland
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£128.00
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Estimated delivery Fri 3 May
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Applications:

WB, IP, ICC, ICC-IF, Flow Cyt

Antibody Host:

Rabbit

Target:

RPA32, Phospho (S4/S8)

Species Reactivity:

Human

Antibody Type:

Recombinant Monoclonal

Immunogen:

surrounding serine 4/8

Alternative Names:

replication factor A protein 2; RPA32; RP-A p34; RP-A p32; RF-A protein 2; replication protein A 32 kDa subunit; REPA2; replication protein A 34 kDa subunit

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Product Description:

Rabbit anti-Phospho RPA32 (S4/S8) Recombinant Monoclonal Antibody [BL-165-5F1] - 20 µl (2 blots)

Storage Temperature:

2 - 8°C

Detection of human Phospho RPA32 (S4/S8)
Detection of human Phospho RPA32 (S4/S8) by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-). Antibodies: Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal antibody [BL-165-5F1] (A700-009-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.
Detection of human Phospho RPA32 (S4/S8)
Detection of human Phospho RPA32 (S4/S8) by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-). Antibodies: Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal antibody [BL-165-5F1] (A700-009-T lot 1) used for IP at 5 µl per reaction. Phospho RPA32 (S4/S8) was also immunoprecipitated by rabbit anti-RPA32 polyclonal antibody (A300-244A). For blotting immunoprecipitated Phospho RPA32 (S4/S8), A700-009-T was used at 1:1000. Chemiluminescence with an exposure time of 3 minutes.
Detection of Human Phospho RPA32 (S4/S8)
Detection of Human Phospho RPA32 (S4/S8) by Immunocytochemistry. Samples: NBF-fixed HeLa cells treated with etoposide (right) or mock treated (left). Antibody: Rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal antibody [BL-165-5F1] (A700-009 lot 1) used at 1:5,000. Detection: DyLight® 594-conjugated goat anti-rabbit IgG (A120-101D4). Counterstain: DAPI (blue).
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