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Rabbit anti-BRD2 Recombinant Monoclonal Antibody [BL-167-2A2]

Catalogue number:
A700-008-T
Size:
20 µl (2 blots)
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£137.00 Estimated delivery Fri 28 Nov
Shipping is calculated in checkout
Applications:

Flow Cyt,IP,WB

Antibody Host:

Rabbit

Target:

BRD2

Species Reactivity:

Human;Mouse

Immunogen:

Between 700 and 750

Alternative Names:

BRD2 intronic transcript 1; BRD2-IT1; bromodomain-containing protein 2; D6S113E; female sterile homeotic-related gene 1; FSH; FSRG1; NAT; O27.1.1; really interesting new gene 3 protein; RING3; RNF3

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Product Description:

Rabbit anti-BRD2 Recombinant Monoclonal Antibody [BL-167-2A2] - 20 µl (2 blots)

Detection of human BRD2 by western blot.
Detection of human BRD2 by western blot. Samples: Whole cell lysate (15 µg) from HEK293T, HeLa, SW620, SK-MEL-28 (SK), Jurkat, MCF-7, Hep-G2 and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD2 recombinant monoclonal antibody [BL-167-2A2] (A700-008-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 75 seconds.
Detection of human BRD2 by western blot
Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRD2 recombinant monoclonal antibody [BL-167-2A2] (A700-008-T lot 1) used for IP at 10 µl per reaction. BRD2 was also immunoprecipitated by rabbit anti-BRD2 antibodies (A302-582A, A302-583A). For blotting immunoprecipitated BRD2, A700-008-T was used at 1:1000. Chemiluminescence with an exposure time of 3 minutes.