CD antigen CD8a, CD8, CD8 antigen, alpha polypeptide (p32), Leu2, Leu2 T-lymphocyte antigen, OKT8 T-cell antigen, p32, T cell co-receptor, T8 T-cell antigen, T-cell antigen Leu2, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation an
A 13 amino acid synthetic peptide from the C-terminal cytoplasmic domain of alpha chain of human CD8 molecule
2 - 8°C
Detection of human CD8 alpha by immunohistochemistry. Sample: FFPE section of human metastatic lymph node. Antibody: Mouse monoclonal anti-CD8 alpha antibody [C8/144B] (A500-021A-T lot 1) used at a dilution of 1:250. Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P). Substrate: DAB.
Detection of human CD8 alpha by immunhistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD8 alpha antibody [C8/144B] (A500-021A-T lot 1) used at 1:100. Secondary: DyLight® 594-conjugated goat anti-mouse IgG (A90-116D4).
Flow cytometric detection of CD8 alpha in human whole blood. Human whole blood was fixed in 4% formaldehyde. Red blood cells were lysed, and the sample was treated with 50% methanol. 1 X 10^6 cells were incubated with 2.5 µl of mouse monoclonal anti-CD8 alpha antibody [C8/144B] (A500-021A-T lot 1) (A) or 0.1 µg mouse IgG1 isotype control (B). Antibody binding was detected with 0.05 µg DyLight® 488-conjugated goat anti-mouse IgG [A90-116D2]. Gated on peripheral blood lymphocytes.