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Mouse anti-BRD2 Monoclonal Antibody, Affinity Purified [12C2E4]

Catalogue number:
A500-014A-T
Size:
20 µl (2 blots) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£128.00 Estimated delivery Fri 26 Apr
Shipping is calculated in checkout
Applications:

WB, IP

Antibody Host:

Mouse

Target:

BRD2

Species Reactivity:

Human

Antibody Type:

Monoclonal

Immunogen:

between 675 and 725

Alternative Names:

D6S113E; RING3; really interesting new gene 3 protein; O27.1.1; NAT; FSRG1; female sterile homeotic-related gene 1; RNF3; bromodomain-containing protein 2; BRD2-IT1; BRD2 intronic transcript 1; FSH

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Product Description:

Mouse anti-BRD2 Monoclonal Antibody, Affinity Purified [12C2E4] - 20 µl (2 blots)

Storage Temperature:

2 - 8°C

Detection of human BRD2 by western blot.
Detection of human BRD2 by western blot. Samples: Whole cell lysate (15 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Mouse monoclonal anti-BRD2 antibody [12C2E4] (A500-014A-T lot 2) used for WB at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P). Chemiluminescence with an exposure time of 3 minutes.
Detection of human BRD2 by western blot
Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Mouse monoclonal anti-BRD2 antibody [12C2E4] (A500-014A-T lot 2) used for IP at 20 µl per reaction. BRD2 was also immunoprecipitated by rabbit anti-BRD2 antibody (A302-582A). For blotting immunoprecipitated BRD2, A500-014A-T was used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P). Chemiluminescence with an exposure time of 3 minutes.
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