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Rabbit anti-MFF Antibody, Affinity Purified

Catalogue number:
A305-638A-T
Size:
10 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£132.00 Estimated delivery Fri 12 Sep
Shipping is calculated in checkout
Applications:

WB, IP

Antibody Host:

Rabbit

Target:

MFF

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

Between 50 and 100

Alternative Names:

C2orf33; mitochondrial fission factor; EMPF2; GL004

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Product Description:

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Detection of human and mouse MFF by west
Detection of human and mouse MFF by western blot. Samples: Whole cell lysate from HeLa (5 and 15 µg) 293T, (50µg), and Jurkat (50µg) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MFF antibody A305-638A-T (lot A305-638A-T-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human MFF by western blot o
Detection of human MFF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T (50µg) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MFF antibody A305-638A-T (lot A305-638A-T-1) used for IP at 20 µl per reaction. MFF was also immunoprecipitated by rabbit anti-MFF antibody A305-629A-T. For blotting immunoprecipitated MFF, A305-638A-T was used at 1:250. Detection: Chemiluminescence with an exposure time of 30 seconds.