The Zymoprep-96 Yeast Plasmid Miniprep kit provides all the necessary reagents for high-throughput plasmid isolation from¬†S. cerevisiae, C. albicans, S. pombe¬†and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The Zymoprep-96 is a simple and efficient yeast plasmid miniprep kit that is based on the classic¬†E. coli¬†alkaline lysis method with our Zymolyase added in the first solution. There is no need for glass beads or phenol. The Zymoprep-96 incorporates our Zymoprep II kit into a 96-well format resulting in a high-throughput method that recovers about 5 fold more plasmid DNA compared to Zymoprep I and eliminates the isopropanol precipitation step. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and hard-to-isolate plasmids. Eluted plasmid DNA can be used directly for E. Coli transformation, PCR, and Southern blot analysis. Includes E1004 x 4: Zymolyase (2 x 2000 Units Lyophilized) w/ Storage Buffer (500µl)]
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