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  2. Bethyl Laboratories, Inc.
  3. A700-007-T
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Rabbit anti-PTPN12 Recombinant Monoclonal Antibody [BL-5-2F8]

Catalogue number:
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A700-007-T
Supplier:
Bethyl Laboratories, Inc.
Size:
20 µl (2 blots)
Product is available in:
  • UK
  • Ireland
  • Europe
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  • Rest of World
£137.00
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Estimated delivery Fri 7 Nov
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Alternative Names:

PTP-PEST; PTPG1; protein-tyrosine phosphatase G1; tyrosine-protein phosphatase non-receptor type 12

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Product Description:

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide

Detection of human PTPN12 by western blo
Detection of human PTPN12 by western blot. Samples: Whole cell lysate (5 µg) from HeLa, HEK293T, Jurkat, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 [SK], and HCT 116 prepared using NETN lysis buffer. Antibody: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (A700-007-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.
Detection of human PTPN12 by western blo
Detection of human PTPN12 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (A700-007-T lot 1) used for IP at 20 µl per reaction. PTPN12 was also immunoprecipitated by rabbit anti-PTPN12 antibody A301-299A. For blotting immunoprecipitated PTPN12, A700-007-T was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.