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Rabbit anti-PTPN12 Recombinant Monoclonal Antibody (BL-5-2F8)

Catalogue number:
A700-007-T
Size:
20 µl (2 blots)
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£105.00 Estimated delivery Fri 8 Jul
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Rabbit anti-PTPN12 Recombinant Monoclonal Antibody (BL-5-2F8) - 20 µl (2 blots)

  • Applications: WB, IP, IHC, ICC
Product Type:

Antibodies

Alternative Names:

protein-tyrosine phosphatase G1, PTPG1, PTP-PEST, tyrosine-protein phosphatase non-receptor type 12

Species Reactivity:

Human, Mouse

Immunogen:

between 499 and 780

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Recombinant Monoclonal

UNSPSC Code:

12352203

Detection of human PTPN12 by western blo

Detection of human PTPN12 by western blot. Samples: Whole cell lysate (5 µg) from HeLa, HEK293T, Jurkat, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 [SK], and HCT 116 prepared using NETN lysis buffer. Antibody: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (A700-007-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.

Detection of human PTPN12 by western blo

Detection of human PTPN12 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (A700-007-T lot 1) used for IP at 20 µl per reaction. PTPN12 was also immunoprecipitated by rabbit anti-PTPN12 antibody A301-299A. For blotting immunoprecipitated PTPN12, A700-007-T was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.

Detection of human PTPN12 by immunohisto

Detection of human PTPN12 by immunohistochemistry. Sample: FFPE section of mammary gland. Antibody: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (A700-007-T lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.

Detection of human PTPN12 by immunohisto

Detection of human PTPN12 by immunohistochemistry. Sample: FFPE section of non-small cell lung cancer. Antibody: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (A700-007-T lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.

Detection of human PTPN12 by immunocytoc

Detection of human PTPN12 by immunocytochemistry. Sample: FFPE section of  SW620 cells. Antibody: Rabbit anti-PTPN12 recombinant monoclonal antibody [BL-5-2F8] (A700-007-T lot 1) used at 1:40. Secondary:HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.

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