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Mouse anti-HIF1-alpha Monoclonal Antibody [H1alpha67]

Catalogue number:
A500-016A-T
Size:
20 µl (2 blots) _$$_
Product is available in:
  • UK
  • Ireland
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  • Rest of World
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Applications:

WB, IP

Antibody Host:

Mouse

Species Reactivity:

Human

Antibody Isotype:

Whole IgG

Antibody Type:

Monoclonal

Immunogen:

Fusion protein containing amino acids 432-528 of human HIF-1 alpha

Alternative Names:

ARNT-interacting protein, basic-helix-loop-helix-PAS protein MOP1, bHLHe78, class E basic helix-loop-helix protein 78, HIF1, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-ALPHA, hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1, alpha subunit (

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Product Description:

The antibody was purified from mouse hybridoma (H1a67) conditioned media.

Storage Temperature:

2 - 8°C

Detection of human HIF1-alpha by western
Detection of human HIF1-alpha by western blot. Samples: Whole cell lysate (50 µg) from Hep-G2 cells treated with 200 µM CoCl2 (+) or mock treated (-). Antibody: Mouse monoclonal anti-HIF1-alpha antibody [H1alpha67] (A500-016A-T lot 1) used for WB at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P). Chemiluminescence with exposure times of 3 minutes.
Detection of human HIF1-alpha by western
Detection of human HIF1-alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 mg for IP, 20% of IP loaded) from Hep-G2 cells with 200 µM CoCl2 (+) or mock treated (-). Antibody: Mouse monoclonal anti-HIF1-alpha antibody [H1alpha67] (A500-016A-T lot 1) used for IP at 6 µl per reaction. For blotting immunoprecipitated HIF1-alpha, A500-016A-T was used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P). Chemiluminescence with exposure times of 3 minutes.
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