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  3. Mouse anti-Rictor Monoclonal Antibody [1G3P2C9]
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Mouse anti-Rictor Monoclonal Antibody [1G3P2C9]

Catalogue number:
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A500-002A-T
Supplier:
Bethyl Laboratories, Inc.
Size:
20 µl (2 blots)
N/A
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This product is no longer available to order.
Applications:

WB, IP, IHC, ICC-IF

Antibody Host:

Mouse

Species Reactivity:

Human

Antibody Isotype:

Whole IgG

Antibody Type:

Monoclonal

Immunogen:

between 1650 and C-term

Alternative Names:

hAVO3; TORC2-specific protein AVO3; rapamycin-insensitive companion of mTOR; PIA; AVO3 homolog; AVO3; pianissimo

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Product Description:

Mouse anti-Rictor Monoclonal Antibody [1G3P2C9] - 20 µl (2 blots)

Detection of human Rictor by western blo
Detection of human Rictor by western blot and immunoprecipitation. Samples: Whole cell lysate [50, 15 and 5 µg for WB; 1 mg for IP, 20% of IP loaded] from 293T cells. Antibodies: Mouse monoclonal anti-Rictor antibody [1G3P2C9] (A500-002A-T lot 1) was used at 1:1000 for WB and 3 µl/mg of lysate for IP. For the above IP reaction, Rictor was immunoprecipitated in lane #1 by A500-002A-T, by rabbit anti-Rictor A300-458A against a disparate epitope in lane #2, and by rabbit anti-Rictor A300-459A against an equivalent epitope in lane #3. Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P). Chemiluminescence with an exposure time of 10 seconds and 3 minutes.
Detection of human Rictor (red) by immun
Detection of human Rictor (red) by immunocytochemistry. Sample: Formaldehyde-fixed HeLa cells. Antibody: Mouse monoclonal anti-Rictor antibody [1G3P2C9] (A500-002A-T lot 1) used at a dilution of 1:1000 and rabbit anti-Rictor antibody (A300-458A) used at a dilution of 1:1000. Secondary: Red fluorescent DuoLink II in situ PLA probes - dual epitope recognition for high specificity.