AVO3, AVO3 homolog, hAVO3, PIA, pianissimo, rapamycin-insensitive companion of mTOR, TORC2-specific protein AVO3
between 1650 and C-term
2 - 8°C
Detection of human Rictor by western blot and immunoprecipitation. Samples: Whole cell lysate [50, 15 and 5 µg for WB; 1 mg for IP, 20% of IP loaded] from 293T cells. Antibodies: Mouse monoclonal anti-Rictor antibody [1G3P2C9] (A500-002A-T lot 1) was used at 1:1000 for WB and 3 µl/mg of lysate for IP. For the above IP reaction, Rictor was immunoprecipitated in lane #1 by A500-002A-T, by rabbit anti-Rictor A300-458A against a disparate epitope in lane #2, and by rabbit anti-Rictor A300-459A against an equivalent epitope in lane #3. Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P). Chemiluminescence with an exposure time of 10 seconds and 3 minutes.
Detection of human Rictor (red) by immunocytochemistry. Sample: Formaldehyde-fixed HeLa cells. Antibody: Mouse monoclonal anti-Rictor antibody [1G3P2C9] (A500-002A-T lot 1) used at a dilution of 1:1000 and rabbit anti-Rictor antibody (A300-458A) used at a dilution of 1:1000. Secondary: Red fluorescent DuoLink II in situ PLA probes - dual epitope recognition for high specificity.