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Mouse anti-Rictor Monoclonal Antibody (1G3P2C9)

Catalogue number:
A500-002A-T
Size:
20 µl (2 blots)
Product is available in:
  • UK
  • Ireland
  • Europe
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  • Rest of World
£105.00 Estimated delivery Fri 8 Jul
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Mouse anti-Rictor Monoclonal Antibody (1G3P2C9) - 20 µl (2 blots)

  • Applications: WB, IP, IHC, ICC-IF
Product Type:

Antibodies

Alternative Names:

AVO3, AVO3 homolog, hAVO3, PIA, pianissimo, rapamycin-insensitive companion of mTOR, TORC2-specific protein AVO3

Species Reactivity:

Human

Immunogen:

between 1650 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Mouse

Antibody Isotype:

Whole IgG

Antibody Type:

Monoclonal

UNSPSC Code:

12352203

Detection of human Rictor by western blo

Detection of human Rictor by western blot and immunoprecipitation. Samples: Whole cell lysate [50, 15 and 5 µg for WB; 1 mg for IP, 20% of IP loaded] from 293T cells. Antibodies: Mouse monoclonal anti-Rictor antibody [1G3P2C9] (A500-002A-T lot 1) was used at 1:1000 for WB and 3 µl/mg of lysate for IP. For the above IP reaction, Rictor was immunoprecipitated in lane #1 by A500-002A-T, by rabbit anti-Rictor A300-458A against a disparate epitope in lane #2, and by rabbit anti-Rictor A300-459A against an equivalent epitope in lane #3. Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P). Chemiluminescence with an exposure time of 10 seconds and 3 minutes.

Detection of human Rictor (red) by immun

Detection of human Rictor (red) by immunocytochemistry. Sample: Formaldehyde-fixed HeLa cells. Antibody: Mouse monoclonal anti-Rictor antibody [1G3P2C9] (A500-002A-T lot 1) used at a dilution of 1:1000 and rabbit anti-Rictor antibody (A300-458A) used at a dilution of 1:1000. Secondary: Red fluorescent DuoLink II in situ PLA probes - dual epitope recognition for high specificity.

Detection of human Rictor by immunohisto

Detection of human Rictor by immunohistochemistry Sample: FFPE section of human prostate carcinoma. Antibody: Mouse monoclonal anti-Rictor antibody [1G3P2C9] (A500-002A-T lot1) used at a dilution of 1:200. Secondary: HRP-conjugated goat anti-mouse IgG (A90-116P). Substrate: DAB.

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