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Rabbit anti-CDK7 Recombinant Monoclonal Antibody [BL-80-5D4]

Catalogue number:
A700-006-T
Size:
20 µl (2 blots) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£128.00 Estimated delivery Wed 3 Apr
Shipping is calculated in checkout
Applications:

WB, IP, IHC, ICC, Flow Cyt

Antibody Host:

Rabbit

Target:

CDK7

Species Reactivity:

Human

Antibody Type:

Recombinant Monoclonal

Immunogen:

between 300 and 346 (C-term)

Alternative Names:

serine/threonine kinase stk1; TFIIH basal transcription factor complex kinase subunit; STK1; Serine/threonine-protein kinase 1; serine/threonine protein kinase 1; p39MO15; p39 Mo15; MO15; kinase subunit of CAK; 39 KDa protein kinase; HCAK; cyclin-dependen

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Product Description:

Rabbit anti-CDK7 Recombinant Monoclonal Antibody [BL-80-5D4] - 20 µl (2 blots)

Storage Temperature:

2 - 8°C

Detection of human CDK7 by western blot.
Detection of human CDK7 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and HCT 116 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CDK7 recombinant monoclonal antibody [BL-80-5D4] (A700-006-T lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.
Detection of human CDK7 by western blot
Detection of human CDK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CDK7 recombinant monoclonal antibody [BL-80-5D4] (A700-006-T lot 1) used for IP at 20 µl per reaction. For blotting immunoprecipitated CDK7, A700-006 was used at 1:500. Chemiluminescence with an exposure time of 30 seconds.
Detection of human CDK7 by immunohistoch
Detection of human CDK7 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-CDK7 recombinant monoclonal antibody [BL-80-5D4] (A700-006-T lot 1) used at a dilution of 1:30. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
Detection of human CDK7 by immunocytoche
Detection of human CDK7 by immunocytochemistry. Sample: FFPE section of human breast carcinoma cell line MCF-7. Antibody: Rabbit anti-CDK7 recombinant monoclonal antibody [BL-80-5D4] (A700-006-T lot 1) used at a dilution of 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Substrate: DAB.
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