www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

loading...
loading...
  1. home
  2. Bethyl Laboratories, Inc.
  3. A700-003-T
*

Rabbit anti-HIF2-alpha Recombinant Monoclonal Antibody [BL-95-1A2]

Catalogue number:
Add to favourites
A700-003-T
Supplier:
Bethyl Laboratories, Inc.
Size:
20 µl (2 blots) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£128.00
add to basket
Estimated delivery Wed 24 Apr
Shipping is calculated in checkout
Applications:

WB, IP, IHC, ICC, ICC-IF, ChIP-Seq

Antibody Host:

Rabbit

Target:

HIF2-alpha

Species Reactivity:

Human

Antibody Type:

Recombinant Monoclonal

Immunogen:

between 400 and 450

Alternative Names:

ECYT4; HIF2-alpha; PAS domain-containing protein 2; MOP2; member of PAS protein 2; Hypoxia-inducible factor 2-alpha; PASD2; hypoxia-inducible factor 2 alpha; HLF; HIF-2-alpha; HIF2A; HIF-1alpha-like factor; HIF-1-alpha-like factor; endothelial PAS domain-

image 0
image 1
image 2
Product Description:

Rabbit anti-HIF2-alpha Recombinant Monoclonal Antibody [BL-95-1A2] - 20 µl (2 blots)

Storage Temperature:

2 - 8°C

Detection of human HIF2-alpha by western
Detection of human HIF2-alpha by western blot. Samples: Whole cell lysate (15 µg) from Hep-G2, HEK293T, and 786-O cells treated with 200 µM CoCl2 (+) or mock treated (-). Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal antibody [BL-95-1A2] (A700-003-T lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with exposure time of 3 minutes.
Detection of human HIF2-alpha by western
Detection of human HIF2-alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Hep-G2 cells with 200 µM CoCl2 (+) or mock treated (-). Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal antibody [BL-95-1A2] (A700-003-T lot 2) used at 20 µl per reaction. For blotting immunoprecipitated HIF2-alpha, A700-003-T was used at 1:1000. Chemiluminescence with exposure time of 3 minutes.
Detection of human HIF2-alpha by immunoh
Detection of human HIF2-alpha by immunohistochemistry. Sample: FFPE section of renal cell carcinoma. Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal antibody [BL-95-1A2] (A700-003-T lot 2) used at 1:400. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Detection of human HIF2-alpha by immunoc
Detection of human HIF2-alpha by immunocytochemistry. Sample: FFPE section of Hep-G2 cells untreated (left) and treated with CoCl2 (right). Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal antibody [BL-95-1A2] (A700-003-T lot 2) used at 1:400. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Detection of human HIF2-alpha by immunoc
Detection of human HIF2-alpha by immunocytochemistry. Sample: Formaldehyde-fixed Hep-G2 cells untreated (left) and treated with CoCl2 (right). Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal antibody [BL-95-1A2] (A700-003-T lot 2) used at 1:100. Secodnasry: DyLight® 594-conjugated goat anti-rabbit IgG (A120-201D4) used at 1:100. Counterstain: Phalloidin Alexa Fluor® 488 conjugated (green).
Localization of HIF2-alpha Binding Sites
Localization of HIF2-alpha Binding Sites by ChIP-sequencing. Chromatin from subcutaneous human tumor 786-O cells was immunoprecipitated with anti-HIF2-alpha antibody A700-003 and analyzed by DNA sequencing. The figure illustrates the peak distribution of HIF2-alpha binding within a 250 Kb region of chromosome 11 as detected using anti-HIF2-alpha antibody A700-003. ChIP-seq validation performed by Active Motif, Carlsbad, CA.
Details Cat number & supplier Size Price
SurePAGE™, Bis-Tris, 10x8, 4-12%, 12 wells M00653 · GenScript Biotech
M00653
GenScript Biotech		 10 pk/box £56.00
10 pk/box
view
SurePAGE™, Bis-Tris, 10x8, 4-20%, 12 wells M00656 · GenScript Biotech
M00656
GenScript Biotech		 10 pk/box £56.00
10 pk/box
view
SurePAGE™, Bis-Tris, 10x8, 10%, 12 wells M00665 · GenScript Biotech
M00665
GenScript Biotech		 10 pk/box £56.00
10 pk/box
view