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  3. A700-001-T
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Rabbit anti-HIF1-alpha Recombinant Monoclonal Antibody [BL-124-3F7]

Catalogue number:
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A700-001-T
Supplier:
Bethyl Laboratories, Inc.
Size:
20 µl (2 blots) _$$_
Product is available in:
  • UK
  • Ireland
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£128.00
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Estimated delivery Fri 26 Apr
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Applications:

WB, IP, IHC, ICC, ChIP-Seq

Antibody Host:

Rabbit

Target:

HIF1-alpha

Species Reactivity:

Human

Antibody Type:

Recombinant Monoclonal

Immunogen:

between 433 and 826

Alternative Names:

HIF1; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); PASD8; PAS domain-containing protein 8; MOP1; member of PAS superfamily 1; member of PAS protein 1; hypoxia-inducible factor1alpha; hypoxia-inducible factor 1-a

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Product Description:

Rabbit anti-HIF1-alpha Recombinant Monoclonal Antibody [BL-124-3F7] - 20 µl (2 blots)

Storage Temperature:

2 - 8°C

Detection of human HIF1-alpha by western
Detection of human HIF1-alpha by western blot. Samples: Whole cell lysate (50 µg) from Hep-G2 and HeLa cells treated with 200 µM CoCl2 (+) or mock treated (-). Antibody: Rabbit anti-HIF1-alpha recombinant monoclonal antibody [BL-124-3F7] (A700-001-T lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.
Detection of human HIF1-alpha by western
Detection of human HIF1-alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 and Hela cells treated with 200 µM CoCl2 (+) or mock treated (-). Antibodies: Rabbit anti-HIF1-alpha recombinant monoclonal antibody [BL-124-3F7] (A700-001-T lot 2) used for IP at 20 µl/mg lysate. For blotting immunoprecipitated HIF1-alpha, A700-001 was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.
Detection of human HIF1-alpha by immunoc
Detection of human HIF1-alpha by immunocytochemistry. Samples: FFPE sections of Hep-G2 cells treated with (left) and without CoCl2 (right). Antibody: Rabbit anti-HIF1-alpha recombinant monoclonal antibody (BL-124-3F7) (A700-001-T lot 1) used at 1:100. Secondary HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Detection of human HIF1-alpha by immunoh
Detection of human HIF1-alpha by immunohistochemistry. Sample: FFPE section of prostate carcinoma. Antibody: Rabbit anti-HIF1-alpha recombinant monoclonal antibody [BL-124-3F7] (A700-001-T lot 1) used at 1:100. Secondary HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Detection of human HIF1-alpha by immunoh
Detection of human HIF1-alpha by immunohistochemistry. Sample: FFPE section of renal cell carcinoma. Antibody: Rabbit anti-HIF1-alpha recombinant monoclonal antibody [BL-124-3F7] (A700-001-T lot 1) used at 1:100. Secondary HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Localization of HIF1-alpha Binding Sites
Localization of HIF1-alpha Binding Sites by ChIP-sequencing. Chromatin from CoCl2 treated Hep-G2 cells was immunoprecipitated with anti-HIF1 alpha antibody A700-001 and analyzed by DNA sequencing. The figure illustrates the peak distribution of HIF1 alpha binding within a 250 Kb region of chromosome 11 as detected using anti-HIF1-alpha antibody A700-001. ChIP-seq validation performed by Active Motif, Carlsbad, CA.
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