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Rabbit anti-BRD4 Recombinant Monoclonal Antibody [BL-149-2H5] - A700-004-T - 20 µl (2 blots) - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-BRD4 Recombinant Monoclonal Antibody [BL-149-2H5]
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Size: 20 µl (2 blots)
£89.00
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Catalogue number:
A700-004-T
Name:
Rabbit anti-BRD4 Recombinant Monoclonal Antibody [BL-149-2H5]
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Recombinant antibody was purified from cell culture supernatant.The epitope recognized by A700-004-T maps to a region between residue 1312 and 1362 of human bromodomain-containing protein 4 using the numbering given in entry NP_490597.1 (GeneID 23476).

Applications:

WB, IP, IHC, ICC-IF, ChIP-Seq

Product Type:
Antibodies
Alternative Names:
bromodomain-containing protein 4, CAP, chromosome-associated protein, HUNK1, HUNKI, MCAP, Protein HUNK1
Species Reactivity:
WB, IP, IHC, ICC-IF, ChIP-Seq
Immunogen:
Between 1312 and 1362
Storage Temp:
2 - 8°C
Antibody Host:
WB, IP, IHC, ICC-IF, ChIP-Seq
Antibody Isotype:
Whole IgG
Antibody Type:
Recombinant Monoclonal
Clone Number:
UNSPSC Code:
12352203
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Detection of human BRD4 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, MDA-MB-231, Hep-G2, A-549, SW620, and SK-MEL-28 (SK) cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (A700-004-T lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.

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Detection of human BRD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (A700-004-T lot 2) used for IP at 20 µl/mg lysate. BRD4 was also immunoprecipitated by rabbit anti-BRD4 antibodies A301-985A and BL5482. For blotting immunoprecipitated BRD4, A700-004-T was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds.

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Detection of human BRD4 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (A700-004-T lot 2) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

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Detection of human BRD4 by immunocytochemistry. Sample: Formaldehyde-fixed HeLa cells. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (A700-004-T lot 2) used at 1:250. Secondary: DyLight® 550-conjugated goat anti-rabbit IgG cross-adsorbed antibody (A120-201D3).

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Localization of BRD4 Binding Sites by ChIP-sequencing. Chromatin from Mia PaCa-2 cells was immunoprecipitated with anti-BRD4 antibody A700-004 and analyzed by DNA sequencing. The figure illustrates the peak distribution of BRD4 binding within a 250 Kb region of chromosome 7 as detected using anti-BRD4 antibody A700-004. ChIP-seq validation performed by Active Motif, Carlsbad, CA.

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