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  2. Bethyl Laboratories, Inc.
  3. A700-000-T
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Rabbit anti-HdmX/MDM4 Recombinant Monoclonal Antibody [BL-3-2F2]

Catalogue number:
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A700-000-T
Supplier:
Bethyl Laboratories, Inc.
Size:
20 µl (2 blots)
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£137.00
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Estimated delivery Fri 7 Nov
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Alternative Names:

MDMX; protein Mdm4; double minute 4, human homolog of; p53-binding protein Mdm4; protein Mdmx; p53-binding protein; MRP1; MDM4 protein variant Y; MDM4 protein variant G; Mdm4 p53 binding protein homolog; HDMX; Double minute 4 protein; mdm2-like p53-bindin

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Product Description:

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide

Detection of human HdmX/MDM4 by western
Detection of human HdmX/MDM4 by western blot. Samples: Whole cell lysate (15 µg) from Jurkat, HEK293T, HeLa, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-HdmX/MDM4 recombinant monoclonal antibody [BL-3-2F2] (A700-000-T lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds.
Detection of human HdmX/MDM4 by western
Detection of human HdmX/MDM4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-HdmX/MDM4 recombinant monoclonal antibody [BL-3-2F2] (A700-000-T lot 2) used for IP at 20 µl/mg lysate. HdmX/MDM4 was also immunoprecipitated by rabbit anti-HdmX/MDM4 antibody BL15229. For blotting immunoprecipitated HdmX/MDM4, A700-000-T was used at 1:1000. Chemiluminescence with an exposure time of 75 seconds.