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Rabbit anti-PolA1 Antibody, Affinity Purified

Catalogue number:
Size:
20 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£468.00 Estimated delivery Wed 24 Sep
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Applications:

WB, IP

Antibody Host:

Rabbit

Target:

PolA1

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

Between 800 and 850

Alternative Names:

POLA; polymerase (DNA-directed), alpha (70kD); polymerase (DNA) alpha 1, catalytic subunit; polymerase (DNA directed), alpha 1, catalytic subunit; NSX; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; p180

Product Description:

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide

Detection of human and mouse PolA1 by we
Detection of human and mouse PolA1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PolA1 antibody A302-850A used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. PolA1 was also immunoprecipitated by rabbit anti-PolA1 antibody A302-851A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).