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  2. Bethyl Laboratories, Inc.
  3. Rabbit anti-BRD2 Antibody, Affinity Purified
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Rabbit anti-BRD2 Antibody, Affinity Purified

Catalogue number:
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A302-582A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
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£436.00
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Estimated delivery Wed 1 May
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Applications:

WB, IP, ICC-IF

Antibody Host:

Rabbit

Target:

BRD2

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

between 1 and 50

Alternative Names:

D6S113E; RING3; really interesting new gene 3 protein; O27.1.1; NAT; FSRG1; female sterile homeotic-related gene 1; RNF3; bromodomain-containing protein 2; BRD2-IT1; BRD2 intronic transcript 1; FSH

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Product Description:

Rabbit anti-BRD2 Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human BRD2 by western blot.
Detection of human BRD2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody A302-582A (lot A302-582A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human BRD2 by western blot
Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody A302-582A (lot A302-582A-2) used for IP at 6 µg per reaction. BRD2 was also immunoprecipitated by a previous lot of this antibody (lot A302-582A-1) and rabbit anti-BRD2 antibody A302-583A. For blotting immunoprecipitated BRD2, A302-582A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human BRD2 by western blot
Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody A302-582A (lot A302-582A-2) used for IP at 6 µg per reaction. BRD2 was also immunoprecipitated by rabbit anti-BRD2 antibody A300-583A and rabbit anti-BRD2 recombinant monoclonal antibody [BL-167-2A2] (A700-008). For blotting immunoprecipitated BRD2, A700-008 was used at 1:1000. Chemiluminescence with an exposure time of 3 minutes.
Detection of human BRD2 by immunocytoche
Detection of human BRD2 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-BRD2 (Cat. No.A302-582A) used at a dilution of 1:100 (2µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 used at a dilution of 1:100.
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