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  2. Bethyl Laboratories, Inc.
  3. Rabbit anti-BRD2 Antibody, Affinity Purified
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Rabbit anti-BRD2 Antibody, Affinity Purified

Catalogue number:
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A302-583A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µl (200 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£436.00
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Estimated delivery Wed 1 May
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Applications:

WB, IP, IHC

Antibody Host:

Rabbit

Target:

BRD2

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

between 675 and 725

Alternative Names:

D6S113E; RING3; really interesting new gene 3 protein; O27.1.1; NAT; FSRG1; female sterile homeotic-related gene 1; RNF3; bromodomain-containing protein 2; BRD2-IT1; BRD2 intronic transcript 1; FSH

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Product Description:

Rabbit anti-BRD2 Antibody, Affinity Purified - 100 µl (200 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human and mouse BRD2 by wes
Detection of human and mouse BRD2 by western blot. Samples:Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody A302-583A (lot A302-583A-5) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human BRD2 by western blot
Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody A302-583A (lot A302-583A-5) used for IP at 6 µg per reaction. BRD2 was also immunoprecipitated by rabbit anti-BRD2 antibody A300-582A and rabbit anti-BRD2 recombinant monoclonal antibody [BL-167-2A2] (A700-008). For blotting immunoprecipitated BRD2, A700-008 was used at 1:1000. Chemiluminescence with an exposure time of 3 minutes.
Detection of human BRD2 by immunohistoch
Detection of human BRD2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD2 (Cat. No.A302-583A Lot5) used at a dilution of 1:200 (1µg/ml). Detection: DAB
Detection of human BRD2 by immunocytoche
Detection of human BRD2 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- BRD2 (Cat. No.A302-583A Lot5) used at a dilution of 1:100 (2µg/ml) (left) and rabbit anti-BRD2 recombinant monoclonal (A700-008-1) used at a dilution of 1:20 (right). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 conjugated.
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