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Rabbit anti-NVL Antibody, Affinity Purified - A304-863A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-NVL Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A304-863A
Name:
Rabbit anti-NVL Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to NVL immobilized on solid support.The epitope recognized by A304-863A maps to a region between residue 75 to 125 of human Nuclear valosin-containing protein-like using the numbering given in entry O15381.1 (GeneID 4931).Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
nuclear valosin-containing protein-like, Nuclear VCP-like protein, NVL2, NVLp
Species Reactivity:
Human
Immunogen:
Between 75 and 125
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human NVL by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NVL antibody A304-863A (lot A304-863A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human NVL by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NVL antibody A304-863A (lot A304-863A-1) used for IP at 6 µg per reaction. NVL was also immunoprecipitated by rabbit anti-NVL antibody A304-864A. For blotting immunoprecipitated NVL, A304-863A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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