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Rabbit anti-TSPYL1 Antibody, Affinity Purified

Catalogue number:
A304-852A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to TSPYL1 immobilized on solid support. The epitope recognized by A304-852A maps to a region between residue 100 to 150 of human Testis-specific Y-encoded-like protein 1 using the numbering given in entry Q9H0U9.3 (GeneID 7259). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

testis-specific Y-encoded-like protein 1, TSPYL, TSPY-like protein 1

Species Reactivity:

Human

Immunogen:

Between 100 and 150

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human TSPYL1 by western blo

Detection of human TSPYL1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TSPYL1 antibody A304-852A (lot A304-852A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human TSPYL1 by western blo

Detection of human TSPYL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TSPYL1 antibody A304-852A (lot A304-852A-1) used for IP at 6 µg per reaction. TSPYL1 was also immunoprecipitated by rabbit anti-TSPYL1 antibody BL18911. For blotting immunoprecipitated TSPYL1, A304-852A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.