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Rabbit anti-POLR1A/RPA194 Antibody, Affinity Purified

Catalogue number:
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£436.00 Estimated delivery Fri 26 Apr
Shipping is calculated in checkout
Applications:

WB, IP

Antibody Host:

Rabbit

Target:

POLR1A/RPA194

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

Between 1425 and 1475

Alternative Names:

RNA polymerase I 194 kDa subunit; DNA-directed RNA polymerase I subunit A; RPO1-4; RPO14; RPA194; RPA1; RNA polymerase I subunit A1; polymerase (RNA) I polypeptide A, 194kDa; A190; DNA-directed RNA polymerase I subunit A1; polymerase (RNA) I subunit A; DN

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Product Description:

Rabbit anti-POLR1A/RPA194 Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human POLR1A/RPA194 by west
Detection of human POLR1A/RPA194 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-POLR1A/RPA194 antibody A304-846A (lot A304-846A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human POLR1A/RPA194 by west
Detection of human POLR1A/RPA194 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-POLR1A/RPA194 antibody A304-846A (lot A304-846A-1) used for IP at 6 µg per reaction. POLR1A/RPA194 was also immunoprecipitated by rabbit anti-POLR1A/RPA194 antibody A304-845A. For blotting immunoprecipitated POLR1A/RPA194, A304-846A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
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