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Rabbit anti-Claspin Antibody, Affinity Purified

Catalogue number:
A300-266A-T
Size:
10 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to Claspin immobilized on solid support. The epitope recognized by A300-266A-T maps to a region between residues 725 and 775 of human Claspin using the numbering given in entry AAG24515.1 (GeneID 63967). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

claspin, claspin homolog, hClaspin

Species Reactivity:

Human

Immunogen:

between 450 and 500

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Claspin by western bl

Detection of human Claspin by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody A300-266A-T (lot A300-266A-T-4) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Claspin by western bl

Detection of human Claspin by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody A300-266A-T (lot A300-266A-T-4) used for IP at 6 µg/mg lysate. For blotting immunoprecipitated Claspin, A300-266A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Claspin by immunohist

Detection of human Claspin by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Claspin (Cat. No. A300-266A-T Lot4) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB