Rabbit anti-Claspin Antibody, Affinity Purified - A300-266A-T - 10 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to Claspin immobilized on solid support. The epitope recognized by A300-266A-T maps to a region between residues 725 and 775 of human Claspin using the numbering given in entry AAG24515.1 (GeneID 63967). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, IHC
Detection of human Claspin by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody A300-266A-T (lot A300-266A-T-4) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human Claspin by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody A300-266A-T (lot A300-266A-T-4) used for IP at 6 µg/mg lysate. For blotting immunoprecipitated Claspin, A300-266A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human Claspin by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Claspin (Cat. No. A300-266A-T Lot4) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
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