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  2. Bethyl Laboratories, Inc.
  3. Rabbit anti-VPS35 Antibody, Affinity Purified
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Rabbit anti-VPS35 Antibody, Affinity Purified

Catalogue number:
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A304-727A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µg
Product is available in:
  • UK
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£468.00
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Estimated delivery Wed 24 Sep
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Applications:

WB, IP

Antibody Host:

Rabbit

Target:

VPS35

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

Between 746 and C-terminus

Alternative Names:

hVPS35; maternal-embryonic 3; MEM3; PARK17; vacuolar protein sorting 35 homolog; vacuolar protein sorting-associated protein 35; Vesicle protein sorting 35

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Product Description:

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Detection of human and mouse VPS35 by we
Detection of human and mouse VPS35 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VPS35 antibody A304-727A (lot A304-727A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human VPS35 by western blot
Detection of human VPS35 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VPS35 antibody A304-727A (lot A304-727A-1) used for IP at 6 µg per reaction. VPS35 was also immunoprecipitated by rabbit anti-VPS35 antibody BL18412. For blotting immunoprecipitated VPS35, A304-727A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.