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  3. Rabbit anti-SPHK1 Antibody Affinity Purified
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Rabbit anti-SPHK1 Antibody Affinity Purified

Catalogue number:
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A302-177A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µl (1000 µg/ml)
Product is available in:
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£468.00
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Estimated delivery Fri 19 Dec
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Applications:

IP,WB

Antibody Host:

Rabbit

Target:

SPHK1

Species Reactivity:

Human

Immunogen:

Between 334 and C-terminus

Alternative Names:

SK 1; sphingosine kinase 1; SPHK; SPK 1

Product Description:

Rabbit anti-SPHK1 Antibody Affinity Purified - 100 µl (1000 µg/ml)

Detection of human SPHK1 by western blot
Detection of human SPHK1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SPHK1 antibody A302-177A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. SPHK1 was also immunoprecipitated by rabbit anti-SPHK1 antibody A302-176A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B). Note: The more slowly migrating bands are believed to represent additional forms of SPHK1 owing to recognition by multiple antibodies each of which was directed toward distinctly different epitopes on SPHK1.