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Rabbit anti-PolH Antibody, Affinity Purified

Catalogue number:
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
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  • Rest of World
£468.00 Estimated delivery Wed 24 Sep
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Applications:

IP

Antibody Host:

Rabbit

Target:

PolH

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

Between 663 and C-terminus

Alternative Names:

RAD30 homolog A; xeroderma pigmentosum variant type protein; XPV; RAD30A; polymerase (DNA) eta; polymerase (DNA directed), eta; DNA polymerase eta; XP-V; RAD30

Product Description:

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Detection of human PolH by western blot
Detection of human PolH by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PolH antibody A301-230A (lot A301-230A-2) used for IP at 3 µg per reaction. PolH was also immunoprecipitated by rabbit anti-PolH antibody A301-231A. For blotting immunoprecipitated PolH, A301-231A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.