Antibody was affinity purified using an epitope specific to USP47 immobilized on solid support. The epitope recognized by A301-049A maps to a region between residue 1237 and the C-terminus (residue 1287) of human ubiquitin specific peptidase 47 using the numbering given in entry NP_060414.3 (GeneID 55031). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
deubiquitinating enzyme 47, Trf (TATA binding protein-related factor)-proximal homolog, TRFP, ubiquitin carboxyl-terminal hydrolase 47, ubiquitin thioesterase 47, ubiquitin thiolesterase 47, ubiquitin-specific-processing protease 47
between 1237 and C-term
2 - 8°C
Detection of human USP47 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP47 antibody A301-049A used for IP at 3 µg/mg lysate. USP47 was also immunoprecipitated by rabbit anti-USP47 antibody A301-048A, which recognizes an upstream epitope. For blotting immunoprecipitated USP47, A301-048A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human USP47 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- USP47 (Cat. No. A301-049A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB