Antibody was affinity purified using an epitope specific to RBM10 immobilized on solid support. The epitope recognized by A301-006A maps to a region between residue 880 and the C-terminus (residue 930) of human RNA binding motif protein 10 using the numbering given in entry NP_005667.2 (GeneID 8241). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DXS8237E, epididymis secretory sperm binding protein, g patch domain-containing protein 9, GPATC9, GPATCH9, RNA-binding motif protein 10, RNA-binding protein 10, RNA-binding protein S1-1, S1-1, TARPS, ZRANB5
between 880 and C-term
2 - 8°C
Detection of human RBM10 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-RBM10 antibody A301-006A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). RBM10 was also immunoprecipitated by rabbit anti-RBM10 antibody BL4415, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).