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Rabbit anti-BubR1 Antibody, Affinity Purified - A300-995A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-BubR1 Antibody, Affinity Purified
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Size: 20 µg
£309.00
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Catalogue number:
A300-995A
Name:
Rabbit anti-BubR1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to BubR1 immobilized on solid support.The epitope recognized by A300-995A maps to a region between residue 1 and 50 of human budding uninhibited by benzimidazoles 1 homolog beta using the numbering given in entry NP_001202.3 (GeneID 701).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
Bub1A, BUB1B, mitotic checkpoint serine/threonine kinase, BUB1beta, BUBR1, budding uninhibited by benzimidazoles 1 homolog beta, hBUBR1, MAD3/BUB1-related protein kinase, MAD3L, mitotic checkpoint kinase MAD3L, mitotic checkpoint serine/threonine-protein
Species Reactivity:
Human
Immunogen:
Between 1 and 50
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human BubR1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody A300-995A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). BubR1 was also immunoprecipitated by rabbit anti-BubR1 antibodies BL1454 and A300-386A, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

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Detection of human BubR1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BubR1 (Cat. No. A300-995A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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