Antibody was affinity purified using an epitope specific to BubR1 immobilized on solid support. The epitope recognized by A300-995A maps to a region between residue 1 and 50 of human budding uninhibited by benzimidazoles 1 homolog beta using the numbering given in entry NP_001202.3 (GeneID 701). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Bub1A, BUB1B, mitotic checkpoint serine/threonine kinase, BUB1beta, BUBR1, budding uninhibited by benzimidazoles 1 homolog beta, hBUBR1, MAD3/BUB1-related protein kinase, MAD3L, mitotic checkpoint kinase MAD3L, mitotic checkpoint serine/threonine-protein
between 1 and 50
2 - 8°C
Detection of human BubR1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody A300-995A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). BubR1 was also immunoprecipitated by rabbit anti-BubR1 antibodies BL1454 and A300-386A, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).