Antibody was affinity purified using an epitope specific to ACC1/ACC-alpha immobilized on solid support. The epitope recognized by A300-986A maps to a region between residue 1925 and 1975 of human acetyl-Coenzyme A carboxylase alpha using the numbering given in entry NP_942131.1 (GeneID 31). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ACAC, ACACAD, ACC, ACC1, ACCA, ACC-alpha, acetyl-CoA carboxylase 1, acetyl-Coenzyme A carboxylase alpha, Biotin carboxylase
between 1925 and 1975
2 - 8°C
Detection of human ACC1/ACC-alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ACC1/ACC-alpha antibody A300-986A used for IP at 3 µg/mg lysate. ACC1/ACC-alpha was blotted using rabbit anti-ACC1/ACC-alpha antibody A300-987A, which recognizes a downstream epitope. For blotting immunoprecipitated ACC1/ACC-alpha, A300-987A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.