Antibody was affinity purified using an epitope specific to SAPS1 immobilized on solid support. The epitope recognized by A300-968A maps to a region between residue 841 and the C-terminus (residue 891) of human SAPS domain family, member 1 using the numbering given in entry NP_055746.2 (GeneID 22870). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
KIAA1115, PP6R1, SAP190, SAPS domain family member 1, SAPS domain family, member 1, SAPS1, serine/threonine-protein phosphatase 6 regulatory subunit 1
between 841 and C-term
2 - 8°C
Detection of human SAPS1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SAPS1 antibody A300-968A used for WB at 0.04 µg/ml (A) and 0.1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). SAPS1 was also immunoprecipitated by rabbit anti-SAPS1 antibody BL4306, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).