Antibody was affinity purified using an epitope specific to RbBP7 immobilized on solid support. The epitope recognized by A300-959A maps to a region between residue 375 and the C-terminus (residue 425) of human Retinoblastoma Binding Protein 7 using the numbering given in entry AAC50231.1 (GeneID 5931). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
G1/S transition control protein-binding protein RbAp46, histone acetyltransferase type B subunit 2, histone-binding protein RBBP7, nucleosome-remodeling factor subunit RBAP46, RbAp46, RBBP-7, retinoblastoma-binding protein 7, retinoblastoma-binding protei
between 375 and C-term
2 - 8°C
Detection of human RbBP7 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RbBP7 antibody A300-959A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). RbBP7 was also immunoprecipitated by rabbit anti-RbBP7 antibody A300-958A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).