Antibody was affinity purified using an epitope specific to EFTUD2/SNRP116 immobilized on solid support. The epitope recognized by A300-957A maps to a region between residue 1 and 50 of human Elongation Factor Tu GTP Binding Domain Containing 2 (U5 snRNP-Specific Protein, 116 kD ) using the numbering given in entry NP_004238.2 (GeneID 9343). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
116 kDa U5 small nuclear ribonucleoprotein component, elongation factor Tu GTP-binding domain-containing protein 2, hSNU114, MFDGA, MFDM, SNRNP116, Snrp116, Snu114, SNU114 homolog, U5 snRNP-specific protein, 116 kDa, U5-116 kDa, U5-116KD
between 1 and 50
2 - 8°C
Detection of human and mouse EFTUD2/SNRP116 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-EFTUD2/SNRP116 antibody A300-957A used for WB at 0.04 µg/ml (A) and 0.1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). EFTUD2/SNRP116 was also immunoprecipitated by rabbit anti-EFTUD2/SNRP116 antibody BL4213, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Detection of human and mouse EFTUD2/SNRP116 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-EFTUD2/SNRP116 (Cat. No. A300-957A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB