Antibody was affinity purified using an epitope specific to KIFC1 immobilized on solid support. The epitope recognized by A300-951A maps to a region between residue 1 and 50 of human Kinesin Family Member C1 (Mitotic Kinesin-Like Protein 2) using the numbering given in entry NP_002254.1 (GeneID 3833). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
HSET, kinesin-like 2, kinesin-like protein 2, kinesin-like protein KIFC1, kinesin-related protein HSET, KNSL2
between 1 and 50
2 - 8°C
Detection of human KIFC1/MKLP2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-KIFC1/MKLP2 antibody A300-951A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). KIFC1/MKLP2 was also immunoprecipitated by rabbit anti-KIFC1/MKLP2 antibodies BL3577, BL3578 and A300-952A, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Detection of human KIFC1/MKLP2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- KIFC1/MKLP2 (Cat. No. A300-951A Lot1) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB