Antibody was affinity purified using an epitope specific to Topo II Beta immobilized on solid support. The epitope recognized by A300-950A maps to a region between residue 1570 and the C-terminus (residue 1621) of human DNA Topoisomerase II Beta using the numbering given in entry NP_001059.2 (GeneID 7155). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
antigen MLAA-44, DNA topoisomerase 2-beta, DNA topoisomerase II, 180 kD, DNA topoisomerase II, beta isozyme, top2beta, TOPIIB, topo II beta, topoisomerase (DNA) II beta 180kDa, topoisomerase II beta, topoisomerase IIb, U937 associated antigen
between 1570 and C-term
2 - 8°C
Detection of human Topo II Beta by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Topo II Beta antibody A300-950A used for WB at 0.04 µg/ml (A) and 0.1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Topo II Beta was also immunoprecipitated by rabbit anti-Topo II Beta antibodies A300-949A and BL4193, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).