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Rabbit anti-Topo II Beta Antibody, Affinity Purified - A300-950A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Topo II Beta Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-950A
Name:
Rabbit anti-Topo II Beta Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Topo II Beta immobilized on solid support.The epitope recognized by A300-950A maps to a region between residue 1570 and the C-terminus (residue 1621) of human DNA Topoisomerase II Beta using the numbering given in entry NP_001059.2 (GeneID 7155).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
antigen MLAA-44, DNA topoisomerase 2-beta, DNA topoisomerase II, 180 kD, DNA topoisomerase II, beta isozyme, top2beta, TOPIIB, topo II beta, topoisomerase (DNA) II beta 180kDa, topoisomerase II beta, topoisomerase IIb, U937 associated antigen
Species Reactivity:
Human
Immunogen:
Between 1570 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Topo II Beta by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Topo II Beta antibody A300-950A used for WB at 0.04 µg/ml (A) and 0.1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). Topo II Beta was also immunoprecipitated by rabbit anti-Topo II Beta antibodies A300-949A and BL4193, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

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Detection of human Topo II Beta by immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti- Topo II Beta (Cat. No. A300-950A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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