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Rabbit anti-DTL/CDT2 Antibody, Affinity Purified - A300-948A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-DTL/CDT2 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-948A
Name:
Rabbit anti-DTL/CDT2 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to DTL/CDT2 immobilized on solid support.The epitope recognized by A300-948A maps to a region between residue 680 and the C-terminus (residue 730) of human Denticleless Homolog (Chromatin Licensing and DNA Replicating Factor 2) using the numbering given in entry NP_057532.1 (GeneID 51514).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
CDT2, DCAF2, DDB1- and CUL4-associated factor 2, denticleless protein homolog, L2DTL, lethal(2) denticleless protein homolog, RAMP, RA-regulated nuclear matrix-associated protein, retinoic acid-regulated nuclear matrix-associated protein
Species Reactivity:
Human, Mouse
Immunogen:
Between 680 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human DTL/CDT2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DTL/CDT2 antibody A300-948A (lot A300-948A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human DTL/CDT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DTL/CDT2 antibody A300-948A (lot A300-948A-2) used for IP at 6 µg per reaction. DTL/CDT2 was also immunoprecipitated by rabbit anti-DTL/CDT2 antibody A300-947A. For blotting immunoprecipitated DTL/CDT2, A300-948A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human DTL/CDT2 by immunohistochemistry.   Sample: FFPE section of human prostate carcinoma.  Antibody: Affinity purified rabbit anti-DTL/CDT2 (Cat. No. A300-948A Lot2) used at a dilution of 1:200 (1µg/ml). Detection: DAB

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Detection of mouse DTL/CDT2 by immunohistochemistry.   Sample: FFPE section of mouse renal cell carcinoma.  Antibody: Affinity purified rabbit anti-DTL/CDT2 (Cat. No. A300-948A Lot2) used at a dilution of 1:200 (1µg/ml).  Detection: DAB

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