Antibody was affinity purified using an epitope specific to DTL/CDT2 immobilized on solid support. The epitope recognized by A300-948A maps to a region between residue 680 and the C-terminus (residue 730) of human Denticleless Homolog (Chromatin Licensing and DNA Replicating Factor 2) using the numbering given in entry NP_057532.1 (GeneID 51514). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CDT2, DCAF2, DDB1- and CUL4-associated factor 2, denticleless protein homolog, L2DTL, lethal(2) denticleless protein homolog, RAMP, RA-regulated nuclear matrix-associated protein, retinoic acid-regulated nuclear matrix-associated protein
Between 680 and C-term
2 - 8°C
Detection of human DTL/CDT2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DTL/CDT2 antibody A300-948A (lot A300-948A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human DTL/CDT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DTL/CDT2 antibody A300-948A (lot A300-948A-2) used for IP at 6 µg per reaction. DTL/CDT2 was also immunoprecipitated by rabbit anti-DTL/CDT2 antibody A300-947A. For blotting immunoprecipitated DTL/CDT2, A300-948A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.