Antibody was affinity purified using an epitope specific to DTL/CDT2 immobilized on solid support. The epitope recognized by A300-947A maps to a region between residue 375 and 425 of human Denticleless Homolog (Chromatin Licensing and DNA Replicating Factor 2) using the numbering given in entry NP_057532.1 (GeneID 51514). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CDT2, DCAF2, DDB1- and CUL4-associated factor 2, denticleless protein homolog, L2DTL, lethal(2) denticleless protein homolog, RAMP, RA-regulated nuclear matrix-associated protein, retinoic acid-regulated nuclear matrix-associated protein
between 375 and 425
2 - 8°C
Detection of human DTL/CDT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DTL/CDT2 antibody A300-947A used for IP at 3 µg/mg lysate. DTL/CDT2 was also immunoprecipitated by rabbit anti-DTL/CDT2 antibodies BL4166, BL4167 and A300-948A, which recognize downstream epitopes. For blotting immunoprecipitated DTL/CDT2, A300-948A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time 10 seconds.
Detection of human DTL/CDT2 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-DTL/CDT2 (Cat. No. A300-947A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB