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Rabbit anti-MYPT1 Antibody, Affinity Purified - A300-888A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-MYPT1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-888A
Name:
Rabbit anti-MYPT1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to MYPT1 immobilized on solid support.The epitope recognized by A300-888A maps to a region between residue 400 and 450 of human Myosin Phosphatase Target Subunit 1 (Protein Phosphatase 1, Regulatory Subunit 12A) using the numbering given in entry NP_002471.1 (GeneID 4659).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
M130, MBS, myosin binding subunit, Myosin phosphatase target subunit 1, myosin phosphatase, target subunit 1, myosin phosphatase-targeting subunit 1, MYPT1, protein phosphatase 1 regulatory subunit 12A, protein phosphatase 1, regulatory (inhibitor) subuni
Species Reactivity:
Human
Immunogen:
Between 400 and 450
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human MYPT1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-MYPT1 antibody A300-888A (lot A300-888A-2) used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate (B). MYPT1 was also immunoprecipitated by a previous lot (lot A300-888A-1) of this antibody and by rabbit anti-MYPT1 antibody A300-889A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

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