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Rabbit anti-JMJD2A Antibody, Affinity Purified - A300-860A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-JMJD2A Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-860A
Name:
Rabbit anti-JMJD2A Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to JMJD2A immobilized on solid support.The epitope recognized by A300-860A maps to a region between residue 350 and 400 of human Jumonji Domain Containing 2A using the numbering given in entry NP_055478.1 (GeneID 9682).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

IP, IHC

Product Type:
Antibodies
Alternative Names:
JHDM3A, jmjC domain-containing histone demethylation protein 3A, JMJD2, JMJD2A, jumonji C domain-containing histone demethylase 3A, jumonji domain containing 2, jumonji domain containing 2A, jumonji domain-containing protein 2A, lysine (K)-specific demeth
Species Reactivity:
Human
Immunogen:
Between 350 and 400
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human JMJD2A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JMJD2A antibody BL3509 (Cat. No. A300-860A) used for IP at 3 µg/mg lysate. JMJD2A was also immunoprecipitated by rabbit anti-JMJD2A antibody BL3513 (Cat. No. A300-861A), which recognizes a downstream epitope. For blotting immunoprecipitated JMJD2A, BL3513 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human JMJD2A by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- JMJD2A (Cat. No. A300-860A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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