Antibody was affinity purified using an epitope specific to JMJD2A immobilized on solid support. The epitope recognized by A300-860A maps to a region between residue 350 and 400 of human Jumonji Domain Containing 2A using the numbering given in entry NP_055478.1 (GeneID 9682). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
JHDM3A, jmjC domain-containing histone demethylation protein 3A, JMJD2, JMJD2A, jumonji C domain-containing histone demethylase 3A, jumonji domain containing 2, jumonji domain containing 2A, jumonji domain-containing protein 2A, lysine (K)-specific demeth
between 350 and 400
2 - 8°C
Detection of human JMJD2A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JMJD2A antibody BL3509 (Cat. No. A300-860A) used for IP at 3 µg/mg lysate. JMJD2A was also immunoprecipitated by rabbit anti-JMJD2A antibody BL3513 (Cat. No. A300-861A), which recognizes a downstream epitope. For blotting immunoprecipitated JMJD2A, BL3513 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human JMJD2A by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- JMJD2A (Cat. No. A300-860A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB