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Rabbit anti-DHX9 Antibody, Affinity Purified - A300-855A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-DHX9 Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-855A
Name:
Rabbit anti-DHX9 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to DHX9 immobilized on solid support.The epitope recognized by A300-855A maps to a region between residue 1220 and the C-terminus (residue 1270) of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 9 using the numbering given in entry CAA71668.1 (GeneID 1660).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
ATP-dependent RNA helicase A, DDX9, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9, DEAH (Asp-Glu-Ala-His) box helicase 9, DEAH (Asp-Glu-Ala-His) box polypeptide 9, DEAH box protein 9, DEAH-box helicase 9, DExH-box helicase 9, leukophysin, LKP, NDH II, ND
Species Reactivity:
Human
Immunogen:
Between 1220 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human DHX9 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX9 antibody A300-855A (lot A300-855A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human DHX9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX9 antibody A300-855A (lot A300-855A-2) used for IP at 6 µg per reaction. DHX9 was also immunoprecipitated by a previous lot of this antibody A300-855A-1 For blotting immunoprecipitated DHX9, A300-855A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human DHX9 by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-DHX9 (Cat. No. A300-855A Lot2) used at a dilution of 1:200 ( 1µg/ml). Detection: DAB

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