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Rabbit anti-DHX9 Antibody, Affinity Purified

Catalogue number:
A300-855A
Size:
20 µg
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Antibody was affinity purified using an epitope specific to DHX9 immobilized on solid support. The epitope recognized by A300-855A maps to a region between residue 1220 and the C-terminus (residue 1270) of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 9 using the numbering given in entry CAA71668.1 (GeneID 1660). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

ATP-dependent RNA helicase A, DDX9, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9, DEAH (Asp-Glu-Ala-His) box helicase 9, DEAH (Asp-Glu-Ala-His) box polypeptide 9, DEAH box protein 9, DEAH-box helicase 9, DExH-box helicase 9, leukophysin, LKP, NDH II, ND

Species Reactivity:

Human

Immunogen:

between 1220 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human DHX9 by western blot.

Detection of human DHX9 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX9 antibody A300-855A (lot A300-855A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human DHX9 by western blot

Detection of human DHX9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX9 antibody A300-855A (lot A300-855A-2) used for IP at 6 µg per reaction. DHX9 was also immunoprecipitated by a previous lot of this antibody A300-855A-1 For blotting immunoprecipitated DHX9, A300-855A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human DHX9 by immunohistoch

Detection of human DHX9 by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-DHX9 (Cat. No. A300-855A Lot2) used at a dilution of 1:200 ( 1µg/ml). Detection: DAB