Antibody was affinity purified using an epitope specific to DHX9 immobilized on solid support. The epitope recognized by A300-854A maps to a region between residue 200 and 250 of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 9 using the numbering given in entry CAA71668.1 (GeneID 1660). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent RNA helicase A, DDX9, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9, DEAH (Asp-Glu-Ala-His) box helicase 9, DEAH (Asp-Glu-Ala-His) box polypeptide 9, DEAH box protein 9, DEAH-box helicase 9, DExH-box helicase 9, leukophysin, LKP, NDH II, ND
between 200 and 250
2 - 8°C
Detection of human DHX9 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DHX9 antibody BL3584 (Cat. No. A300-854A) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B). DHX9 was immunoprecipitated by rabbit anti-DHX9 antibody BL3587 (Cat. No. A300-855A), which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).