Antibody was affinity purified using an epitope specific to USP34 immobilized on solid support. The epitope recognized by A300-824A maps to a region between residue 3350 and the C-terminus (residue 3395) of human Ubiquitin Specific Protease 34 using the numbering given in entry CAE51938.1 (GeneID 9736). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
deubiquitinating enzyme 34, ubiquitin carboxyl-terminal hydrolase 34, ubiquitin specific protease 34, ubiquitin thioesterase 34, ubiquitin thiolesterase 34, ubiquitin-specific-processing protease 34
between 3350 and C-term
2 - 8°C
Detection of human and mouse USP34 by WB (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-USP34 antibody BL2158 (Cat. No. A300-824A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). USP34 was also immunoprecipitated by rabbit anti-USP34 antibody BL2157, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).