Antibody was affinity purified using an epitope specific to CRSP1/TRAP220 immobilized on solid support. The epitope recognized by A300-793A maps to a region between residue 1525 and the C-terminus (residue 1581) of human Cofactor Required for Sp1 Transcriptional Activation 1 (Thyroid Hormone Receptor-Associated Protein Complex Component TRAP220) using the numbering given in entry NP_004765.2 (GeneID 5469). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
activator-recruited cofactor 205 kDa component, ARC205, CRSP1, CRSP200, DRIP205, DRIP230, Mediator complex subunit 1, mediator of RNA polymerase II transcription subunit 1, p53 regulatory protein RB18A, PBP, peroxisome proliferator-activated receptor-bind
between 1525 and C-term
2 - 8°C
Detection of human and mouse MED1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MED1 antibody A300-793A (lot A300-793A-9) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human MED1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MED1 antibody A300-793A (lot A300-793A-9) used for IP at 6 µg per reaction. MED1 was also immunoprecipitated by tabbit anti-MED1 recombinant monoclonal antibody [BLR037F] (A700-037). For blotting immunoprecipitated MED1, A700-037 was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.